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KMID : 0359320090490010017
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Korean Journal of Veterinary Research
2009 Volume.49 No. 1 p.17 ~ p.22
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The effect of Bu-Zhong-Yi-Qi-Tang on ultraviolet B-induced skin damages in mouse
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Kim Seong-Ho
Jo Sung-Kee
Kim Joong-Sun
Song Myoung-Sub
Moon Chang-Jong
Lee Hae-June
Jo Sung-Kee
Bae Chun-Sik
Kim Jong-Choon
Seo Heung-Sik
Lee Hae-June
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Abstract
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The effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1-hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/cm2) and were sacrificed 24 h later. BZYQT (50 mg/kg of body weight) or vehicle (saline) was given i.p. at 36 and 12 h before irradiation, and 30 min after irradiation or BZYQT cream (0.2%) or cream base (vehicle) was topically treated at 24 h and 15 min before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/cm length of epidermis, and 24 h after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was reduced by intraperitoneal injection of BZYQT extract (p < 0.01). The numbers of DC in normal ICR mouse were 628.00 ± 51.56 or 663.20 ± 62.58 per mm2 of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/mm2 were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. Treatment of BZYQT was associated with increase of 33.9% in i.p. group (p < 0.05) or 2.7% in topical application group in the number of ATPase positive cells compared with the irradiation control group. The results presented herein that BZYQT administration could reduce the extent of skin damages produced by UVB.
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KEYWORD
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ATPase-positive dendritic cell, Bu-Zhong-Yi-Qi-Tang, sunburn cell, ultraviolet B
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